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PSEUDOMONAS FLUORESCENS |
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Formulation |
3x1011CFU/G WP, 5x108 CFU/G GR |
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Shelf Life |
12 months. |
Core Summary
Fluorescent Pseudomonas belongs to the genus Pseudomonas and is a Gram-negative, rod-shaped bacterium. Its most notable feature is that it can produce an iron-loving substance that emits yellow-green fluorescence under ultraviolet light in an iron-deficient environment, hence the name "fluorescent" Pseudomonas. It is a rhizosphere colonizing probiotic, and its life activities are closely centered around the plant root system.
Mechanism of Action (Multifunctionality)
Similar to the spore-forming Bacillus multivarius, Fluorescent Pseudomonas also functions through multiple mechanisms, but it has its own unique focuses and methods:
Competitive effect (core mechanism)
Iron competition: This is one of its most important antagonistic mechanisms. In the soil, plant pathogens (such as Fusarium, Rhizoctonia) also need iron elements to grow. Pseudomonas fluorescens can secrete efficient siderophores, binding to the iron ions (Fe³⁺) in the environment first and bringing them into its own body. This causes the pathogens to be unable to grow and reproduce normally due to "iron deficiency", thereby being inhibited.
Root colonization site competition: It can rapidly multiply on the plant root surface and in the rhizosphere soil, forming an advantageous bacterial community, physically occupying the "gate" through which pathogen invasion occurs.
Antagonistic effect
In addition to iron competition, it can also produce various antibiotics and antibacterial substances, directly inhibiting pathogenic bacteria:
Antibiotics: Such as Tenuigranulose, 2,4-diacetyl tenuifolin, etc., have inhibitory effects on various fungal and bacterial pathogens.
Hydrolytic enzymes: Secretion of chitinase, glucanase, etc., dissolving the cell walls of fungi.
Hydrogen cyanide: Toxic to certain pathogenic bacteria and nematodes.
Induction of systemic resistance
It can systematically activate the jasmonic acid/ethylene-dependent signaling pathways in plants, putting the plants as a whole in a "warning state", enhancing the resistance to subsequent pathogen invasion. This is a long-lasting immune mechanism.
Probiotic effect
Producing plant hormones: Synthesis of auxins such as indole-3-acetic acid, stimulating root development, increasing the number of adventitious roots and root hairs.
Improving nutrient absorption: Through acidifying the environment, secreting chelators, etc., activating the insoluble mineral elements such as phosphorus, iron, manganese, and zinc in the soil, allowing plants to absorb them.
Regulating plant ethylene levels: Through the production of ACC deaminase, reducing the stress ethylene produced by plants under adverse conditions (such as drought, salinity), alleviating stress and promoting growth.
Main Target Diseases and Applications
● Fluorescent Pseudomonas mainly targets soil-borne diseases, especially root diseases caused by fungi.
● Fungal diseases: Blight, damping-off. Wilting, yellowing. Root rot.
● Bacterial diseases: It also has a certain inhibitory effect on some bacterial diseases (such as bacterial wilt).
● Nematodes: It has a certain repellent and inhibitory effect on plant parasitic nematodes.
● Application methods:
○ Soil drenching/drip irrigation: The primary application method, ensuring the application of the microbial agent reaches the root system.
○ Seed treatment: Coating or mixing with seeds, establishing a protective ring when the seeds germinate.
○ Seedling bed treatment: Providing a healthy rhizosphere environment for young seedlings.
Key Features
Strong root zone colonization ability
It can quickly adapt to the root zone environment, utilize root exudates for reproduction, and move along with the growth of the root system to form a dynamic protective layer.
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High efficiency in iron competition
The siderophore mechanism is its unique and powerful weapon that distinguishes it from other biocontrol bacteria.
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Significant promoting effect
Not only can it prevent diseases, but it can also directly promote crop growth, achieving the dual goals of "preventing diseases and increasing yield".
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Good environmental compatibility
It is part of the native microbial community in the soil and is safe and pollution-free.
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Usage Notes
Strictly avoid mixing with chemical disinfectants: especially copper-based and broad-spectrum disinfectants, which will directly kill the bacteria.
Use in advance and continuously: It is a preventive agent and must be used before the rice disease occurs to exert its effect by establishing a dominant population.
Pay attention to environmental conditions: Soil moisture, temperature, and organic matter content will affect its colonization effect. Maintaining soil moisture is beneficial for its reproduction.
Product quality is crucial: The number of live bacteria and the activity of the strain are the key to the effectiveness.
Storage and transportation: Store in a dark place at 2-8℃; prevent high temperatures during transportation.
Shelf life: 12 months (live bacterial preparation)
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