Metarhizium Anisopliae Nematicide

Metarhizium Anisopliae Nematicide

Metarhizium anisopliae Nematicide is a broad-spectrum biological nematicide based on concentrated fungal conidia, specifically isolated to control destructive plant-parasitic nematodes in agricultural soils.
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Description
Technical Parameters

Metarhizium anisopliae Nematicide is a broad-spectrum biological nematicide based on concentrated fungal conidia, specifically isolated to control destructive plant-parasitic nematodes in agricultural soils. This microbial solution penetrates insect and nematode cuticles, delivering biological control without chemical residues or resistance buildup. Formulated for high physical stability, it integrates directly into soil application equipment, drip irrigation systems, and commercial fertilizer blends.

 

Active Microorganism

 

 

Strain

Metarhizium anisopliae

Active State

Pure fungal conidiospores

Standard Potency

1 × 10¹⁰ CFU/g (Wettable Powder) / 2 × 10¹⁰ CFU/g (Technical Powder)

Biological Origin

Naturally occurring entomopathogenic and nematophagous fungus

Purity Rate

99.5% minimum (Free of plant pathogens and non-target microbial contaminants)

 

Biological Mode of Action

 

 

Adhesion and Recognition: Conidia attach to the outer cuticle of juvenile nematodes and nematode eggs in the rhizosphere.


Germination and Enzymatic Digestion: Under suitable moisture conditions, conidia germinate and produce specialized appressoria that secrete extracellular enzymes, including chitinases, proteases, and esterases, to break down the nematode cuticle.


Hyphal Penetration: Infection hyphae penetrate directly through the protective outer membrane into the body cavity.


Internal Colonization: Fungal mycelia proliferate inside the host, absorbing internal nutrients, disrupting metabolic functions, and suppressing egg hatching.


Sporulation: Upon host death, new conidia emerge on the external surface of the nematode carcass, dispersing into surrounding soil matrix to provide persistent suppressive activity.

 

Target Nematodes

 

Root-Knot Nematodes

Effective against active juveniles (J2 stage) and egg masses of root-knot species, preventing root galls and vascular damage.

Meloidogyne Species

  • Meloidogyne incognita (Cotton Root-Knot Nematode)
  • Meloidogyne hapla (Northern Root-Knot Nematode)
  • Meloidogyne javanica (Javanese Root-Knot Nematode)
  • Meloidogyne arenaria (Peanut Root-Knot Nematode)

Other Plant-Parasitic Nematodes

  • Cyst Nematodes (Heterodera species, Globodera species)
  • Lesion Nematodes (Pratylenchus species)
  • Reniform Nematodes (Rotylenchulus reniformis)
  • Dagger Nematodes (Xiphinema species)

 

Crops and Applications

 

 

Solanaceous Crops (Tomato, Pepper, Eggplant): Apply through root drenching or drip systems during seedling transplanting to protect early root architecture.

 

Cucurbits (Cucumber, Melon, Squash): Inject via fertigation prior to vine growth to control soil-dwelling juveniles.

 

Root and Tuber Crops (Potato, Carrot, Sweet Potato): Incorporate into bed prep or soil broadcast before planting to minimize surface damage and galling.

 

Perennial Fruit Trees and Vineyards (Citrus, Grape, Apple): Apply during seasonal root flushes via micro-sprinklers to suppress established sub-surface populations.

 

Protected Horticulture (Greenhouse Vegetables and Ornamentals): Mix with growing media or apply through recirculating irrigation systems for continuous rhizosphere protection.

 

Product Specifications

 

 

Parameter

Specification (Technical Grade)

Specification (Wettable Powder)

Active Ingredient

Metarhizium anisopliae conidia

Metarhizium anisopliae conidia

Appearance

Dark green to olive powder

Light green fine powder

Spore Count

2.0 × 10¹⁰ CFU/g minimum

1.0 × 10¹⁰ CFU/g minimum

Spore Germination Rate

90.0% minimum (after 24h at 25 degrees C)

88.0% minimum (after 24h at 25 degrees C)

Moisture Content

5.0% maximum

6.0% maximum

Particle Size

100% through 80 mesh

98% through 325 mesh

Suspension Rate

Not Applicable

85.0% minimum

pH Range

5.5 to 7.5

5.5 to 7.5

Contaminant Microbes

Below 0.1%

Below 0.1%

 

Formulation and Packaging

 

 

Formulation Types: Technical Powder, Wettable Powder, Oil Dispersion, Granule for soil incorporation.


Standard Bulk Packaging: 1 kg aluminum foil bags (10 bags per carton), 25 kg fiber drums with double-layer inner polyethylene liners, 500 kg bulk tote bags for granular forms.


Customization Options: Private labeling, tailored spore concentrations, specific package sizing, and nitrogen-flushed barrier packaging.

 

Technical Documentation

 
01/

Certificate of Analysis: Issued per production batch showing CFU count, germination rate, and purity metrics.

02/

Safety Data Sheet: Standardized document compliant with global hazard communication rules.

03/

Five-Batch Analysis Report: Complete characterization data available for technical registration procedures.

04/

Product Data Sheet: Technical guidance covering tank mixing, biological compatibility, and storage conditions.

05/

Ecotoxicology Studies: Full toxicological profiles covering mammalian safety, avian safety, aquatic safety, and non-target beneficial insect assessments.

Supply and Delivery

 

 

Annual Production Capacity: 1,200 Metric Tons across standardized solid-state fermentation lines.


Quality Management: Manufacturing facilities operating under strict batch traceability systems from mother culture to final packaging.


Minimum Order Quantity: 100 kg for standard formulations; 500 kg for custom formulations or private packaging.


Production Lead Time: 10 to 15 business days for standard inventory; 20 to 25 business days for custom orders.


Cold-Chain Shipping: Temperature-controlled container freight options (4 to 15 degrees C) available to maintain spore viability during long-distance transit.

 

FAQ

 

 

Q: What is the shelf life of Metarhizium anisopliae Nematicide, and how should it be stored?

A: Stored in unopened original moisture-barrier packaging at cool temperatures (2 to 8 degrees C), the product maintains stated CFU levels for 12 months. At ambient room temperature (20 to 25 degrees C), shelf life is 6 months. Keep in a dry place protected from direct sunlight.

Q: Is Metarhizium anisopliae Nematicide compatible with chemical fungicides and fertilizers?

A: It can be tank-mixed with most organic fertilizers, biostimulants, non-bactericidal soil amendments, and insecticides. Avoid direct mixing with systemic broad-spectrum fungicides, copper compounds, or strong alkaline solutions. Allow a minimum 7-day interval between synthetic fungicide treatments and product application.

Q: How does Metarhizium anisopliae Nematicide perform through drip irrigation systems without clogging lines?

A: The Wettable Powder formulation undergoes specialized micro-grinding and air-jet milling to achieve a particle size where 98% passes through a 325-mesh screen. It exhibits rapid dispersibility and a suspension rate exceeding 85%, ensuring smooth passage through standard drip emitters, line filters, and dosing equipment.

Q: What regulatory registration support is available for international importers?

A: Full technical dossier support is provided, including Certificate of Analysis, Safety Data Sheet, product specifications, manufacturing flow charts, toxicology summaries, and five-batch analysis reports to assist with local biological pesticide registrations.

Q: What application timing yields the highest biological efficiency against root-knot nematodes?

A: Application should occur before or during planting, seeding, or transplanting when soil temperatures exceed 15 degrees C. Pre-transplant soil treatment allows conidia to establish in the root zone prior to nematode hatch cycles, protecting primary roots during early plant establishment.

 

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